FORMULATION AND EVALUATION OF PHARMACEUTICALY STABLE SERUM OF DUCHESNEA INDICA (ARBENAN)

Objective: Arbenan ( Duchesnea indica ) plants contain saponins, flavonoids, and tannins which have antioxidant activity. The purpose of this research is to perform formulation and evaluation extract ethanol of Arbenan leaves in the form of serum which is pharmaceutically stable. Method: Arbenan leaf powder was macerated with ethanol solvent, and then left for 3-4 days while stirring repeatedly, and then filtering. Furthermore, the liquid ethanol extract that has been obtained is evaporated using a Rotary Vacum Evaporator was used to evaporate the extract. Prepared extract was used to evaluate various parameters like organoleptics, homogeneity, viscosity, and pH. Result: All formulations were having typical smell, light brown color and a little thick consistency. Formulations of leaf extract of Arabenan with four variations bases have shown to have good stability after stress condition. It can be seen from the evaluation result are organoleptics, homogeneity, viscosity, rheology, and pH. Conclusion: Study concludes that a stable leaf extract of Arabenan can be effectively formulated into a serum by the means of various bases.


INTRODUCTION
Molecular damage in the body can be induced by molecules called free, radicals 7 . Free radicals can be formed due to internal and external sources of free radicals 1 . Internal sources of free radicals are factors derived from normal metabolite processes in the human body, namely phagocytes, xanthine oxidase, arachidonic pathways, peroxisomes, inflammation and others. External sources of free radicals are factors that originate outside the human body, namely cigarette smoke, environmental pollution, sunlight, chemicals, ozone, several types of drugs, pesticides and others. Excessive levels of free radicals are a trigger for various degenerative diseases and conditions 4 . Antioxidants can inactivate the development of oxidation reactions by binding to free radicals and highly reactive molecules so that cell damage can be inhibited 2,3, 4 . The use of natural materials that have biological activity is the motivation for further research, after synthetic compounds that have biological activity such as synthetic antioxidant compounds Butylated Hydroxyanisole (BHA) and Butylated Hydroxytoluene (BHT) are restricted because they are carcinogenic 1 .There is concern about the possible side effects of synthetic antioxidants causing natural antioxidants to become alternatives that need to be developed 5 . One of the plants that can be used as medicine is a plant (Duchesnea indica (Jacks.) Focke) known as Arbenan. The community uses this plant as a fever, anti-infection and stimulant 18 . In addition, Arbenan is also used for cancer treatment, anti-inflammatory, stop bleeding, destroy blood clots, and reduce swelling 6 . Flavonoids are compounds that have antioxidant activity due to the presence of a hydroxy group in their molecular structure so they are called bioflavonoids. Likewise, some tannins have been shown to have antioxidant activity, inhibit tumor growth and inhibit enzymes such as reverse transcriptase and DNA topoisomerase. While some saponins work as antimicrobials 3 . However, the use of Arbenan as an Serum is a preparation that has more bioactive components. Serum has the advantage that it can provide a more comfortable effect and is easier to spread on the skin surface because its viscosity is not too high 7, 8 . Serum or called concentrate, contains ten times more biologically active substances than cream preparations, so it is faster and more effective. Serum has fast absorption properties and the ability to penetrate into the deeper layers of the skin. The selection of serum preparations is motivated by the form of preparation that is easy to make, practical to use, easily absorbs into the skin and gives a soft and moist feeling after use 8 . Serum works locally on different parts of the body, face, neck, eyelids. This preparation can be used regardless of age 9, 10 . Based on the explanation above, a research will be conducted on the formulation and evaluation of the ethanol extract serum of Arbenan [Duchesnea indica (Jacks.) Focke] leaves which are pharmaceutically stable. This research was conducted to made serum preparations that are practical to use and provide antioxidant activity that can maintain its stability on storage. The use of the percentage of the extract in a formula based on IC50 Arbenan leaf ethanol extract was 30.20 µg/mL 15 . An increase in the extract concentration was carried out up to 100x times that of the IC50 in order to qualify as a serum, namely a highly concentrated skin preparation 11,12 .

Preparation Sample
The collected samples of Arbenan were cleaned of dirt adhering to the leaves using running water and then dried by aerating. After drying the sample is then mashed 13 .

Extraction Method
Arbenan leaf powder is weighed as much as 300 grams, put in a maceration container then 2700 mL of 70% ethanol solvent is added until the sample is submerged, then left for 3-4 days while stirring repeatedly, then filtering and obtaining residual and liquid ethanol extract. Furthermore, the liquid ethanol extract that has been obtained is evaporated using a Rotary Vacum Evaporator to obtain a thick ethanol extract. The use of extract percentage in formulas based on IC50 Arbenan leaf ethanol extract is 30.20µg/mL 14 . Increase the concentration of the extract to 100x times that of IC 50 in order to qualify as a serum with high concentrated skin preparations.

Preparation of serum ethanol extract of Arbenan leaf
Prepared tools and materials. Weigh all ingredients to be used. The suitable basic formulation is selected from the optimization results. The base is dispersed in heated aquadest and added with propyl paraben and methyl paraben which have been dissolved in propylene glycol, added tocopherol then homogenized 15 .

Characterization of serum extract of Arbenan Leaf Organoleptic
Organoleptic tests are performed visually on serum preparations which include shape, color, and smell 13 .

Homogeneity
The preparation is placed between two glass slides and then the presence of coarse particles or inhomogeneity under the light is observed 16 .

Measurement of Viscosity and Flow Properties
Viscosity measurements were carried out using a Brookfield viscometer. The preparation is put into a measuring cup then the appropriate spindle is lowered until the spindle limit is immersed into the preparation. Then the motor and spindle are started. Player speed is set 0.5 successively; 2; 5; 10; and 20 rpm is then reversed from 20; 10; 5; 2; and 0.5 rpm. The viscosity number indicated by the red needle is noted. Then it is multiplied by the correction factor in the table on the tool brochure 17 .

Stability Test
Evaluation of the stability of the preparation is carried out before and after the conditions are imposed. The condition was enforced by storing the preparation as much as 100.00 mL at a temperature of 5C and 35C alternately for 12 hours each for 10 cycles.

Deployment Ability
A total of 0.5 mL of the preparation was placed on a diameter of 15 cm round glass, another glass was placed on it and allowed to stand for 1 minute. Then, a 50 gram load is added and allowed to stand for 1 minute and then a constant diameter of 5-7 cm is measured, showing a semisolid consistency which is very comfortable to use 18 . The pH examination of serum preparations was carried out before and after the stress condition using a pH meter. The pH meter is immersed into the serum preparation to the limit of the mark and the pH value of the serum preparation will be read 12 .

Tabel 2:
The results of the measurement of the different parameters of the Arabenan leaf extract serum formula before and after the conditions were imposed.

RESULT AND DISCUSSION
Molecular damage in the body can be induced by molecules called free radicals 11 . Free radicals can be formed due to internal and external sources of free radicals. Excessive levels of free radicals are a trigger for various degenerative diseases and conditions 4 . Antioxidant can be prevents or prevents oxidation, or natural or synthetic substances. Antioxidants can inactivate the development of oxidation reactions by binding to free radicals and highly reactive molecules so that cell damage can be inhibited. Synthetic compounds that have biological activity such as synthetic antioxidant compounds Butylated Hydroxyanisole (BHA) and Butylated Hydroxytoluene (BHT) because of their use because they are carcinogenic 9 . Therefore, natural antioxidants are an alternative that needs to be developed. One of the uses of natural materials that have biological activity is Arbenan plants. All parts of the Arbenan plant contain saponins, flavonoids, and tannins. Flavonoids are compounds that have antioxidant activity due to the presence of a hydroxy group in their molecular structure so they are called bioflavonoids. However, the use of Arbenan as an antioxidant for skin is not widely known by the public. Aesthetic use certainly does not provide comfort. Therefore it is necessary to develop Arbenan as a serum preparation. Serum dosage forms are easy to make, practical to use, easy to penetrate into the skin and provide a soft and moist feeling after use. Besides, it can provide a more comfortable effect and spread more easily on the surface of the skin because the viscosity is not too high 10,17 . The extract was made by maceration method using 70% ethanol as the solvent. In the formulation, the HPMC and Na CMC bases were optimized. As for the variation, the concentration of HPMC used was 0.5% and 1%, while the Na CMC base used concentrations of 0.5% and 1%. This variation is carried out to optimize the basis that is effective to meet the criteria for pharmaceutical sedioan physical properties and can survive the shelf life through stability testing. Stability testing is carried out using the stress condition method using a climatic chamber. The tested preparations were stored at 5C and 35C for 10 cycles, each cycle lasting 12 hours. The purpose of this test is to determine the physical stability of the preparation. The resulting formulation was then evaluated pharmaceutically. All formulations were having typical smell, light brown color and a little thick consistency. The homogeneity test was carried out to see a homogeneous serum composition. The composition of the serum is said to be homogeneous if there is an even color equation and no different particles are found 10 . Homogeneity observations were carried out visually with a glass object, where smearing the serum sample on a glass object was observed. From the results of testing the homogeneity of F1, F2, F3 and F4 before and after the forced conditions on the serum formula of Arabenan leaf extract showed that the formula was homogeneous which was marked by the absence of coarse particles in the preparation. Viscosity testing aims to determine the consistency of preparations that affect the skin. The higher the viscosity value, the more difficult it is to apply to the skin, the lower the viscosity, the easier it is to apply to the skin. The factors that affect viscosity are pressure, temperature, size and molecular weight 11 . The results obtained that the serum using HPMC had a higher viscosity than that of Na CMC. In measuring the viscosity of Arabenan leaf extract serum, a Brookfield Viscometer was used. The viscosity of the preparation was measured using a spindle number 62 with the rotating speed adjusted to 0.5 successively; 2; 5; 10; and 20 rpm is then reversed from 20; 10; 5; 2; and 0.5 rpm for four replications. The results obtained can be seen in Table 2. The viscosity of the preparations before and after the stress condition there was a change in the decrease in the mean viscosity at F1, F2, F3 and F4. This may be because in the formulation there are variations in the concentration of the base used to improve the appearance of the preparation. The viscosity data obtained were analyzed statistically using the One-Way ANOVA method. The results of the analysis can be seen in Table 2, which shows that for serum preparations, the viscosity of all formulas. There was slight significant change in the conditions. This suggests that the existence of a forced condition greatly affects the viscosity of all norms.   In determining the flow type of Arabenan leaf extract serum, a Brookfield Viscometer was also used. The type of flow can be seen from the rogram and the yield value of the preparation. The yield value is the price that must be met in order for the preparation to flow 11 . The yield value is obtained from measuring the viscosity of the preparation at several rpm, then the data obtained can be determined by shearing stress and shear speed (rate of share). After creating a rheogram linking the shearing stress and the shear speed (rate of share), the newton flow type is obtained which is formed from the four formulas, namely plastic flow. It is said to be plastic flow because the results of the rogram show that the rising and falling curves cut the yield value in the absence of a hysteresis loop. Flow types in F1, F2, F3 and F4 did not change the flow both before and after the accelerated condition. The results obtained can be seen in the rheogram. The spreadability test is carried out to determine how much the spreadability of the serum is, because it is a good preparation and is preferable if it can spread easily and is comfortable to use 17 . The greater the dispersibility value, the easier the serum will spread on the skin. From the test results the spreadability is inversely proportional to the viscosity of a preparation, the thicker the consistency, the smaller the dispersion power produced. The value of the scattering power whose consistency is very comfortable to use is 5-7 cm 10 . The results of the measurement of the scattering power can be seen in Table 2. From the test results, the spreadability did not meet the good serum dispersion parameters. Literature studies were carried out in several journal base formulations with HPMC and Na CMC as the basis. Research on serum formulations with HPMC and Na CMC according to Shukr and Metwally (showed that the dispersibility produced by 0.5% and 1% HPMC and Na CMC concentrations of gel was 8-15 cm. In the Mappa study it was also said that the dispersibility. The gel is not good because the viscosity of Na CMC is too high. When Na CMC is put into water, Na + is released and replaced with H + ions and forms HCMC which will increase the viscosity 15 and Na CMC also determines the viscosity stability and spreadability of the gel preparation so that further research is needed regarding the effect of differences in the concentration of additional strength of the gel on physical stability, with this study it is necessary to adjust the formula. After the spreadability test, pH measurements were carried out which aims to see whether the pH on the preparation matches the pH on the skin. The pH measurement of the preparation was carried out before and after the conditions were imposed. This is related to the problem of stability and safety of using preparations to avoid irritation of the skin for its users, the pH of skin preparations should have a pH that is approximately the same as the pH of the skin, which is between 5-7 12 . The pH measurement results can be seen in Table 2. From the results of pH measurement, the Arabenan leaf extract serum formula before and after the stress condition to meet the requirements because it had a pH between 5.6 to 6.17. A stable pH indicates that the components in the preparation are still in the pH range category and are not affected by temperature so that the preparation remains stable during storage. So that the results of pH measurements for each formula 1 and 2 on the basis of HPMC and formulas 3 and 4 on the basis of Na CMC are concluded to remain stable and safe to use. Even though the pH has decreased and increased after the conditions are imposed, the indicated pH changes are very small and still acceptable because they still meet the pH range of the preparation for the skin.