GC-MS ANALYSIS OF FIXED OILS OF NIGELLA SATIVA SEEDS

  • Sania Feroz Institute of Chemical Sciences, University of Peshawar, K.P.K Peshawar-25120, Pakistan
  • Ghias Uddin Institute of Chemical Sciences, University of Peshawar, K.P.K Peshawar-25120, Pakistan

Abstract

Gas chromatography-Mass spectrometry (GC-MS) is an analytical method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, fire investigation, environmental analysis, explosives investigation, and identification of unknown samples. The present study also relies on use of GC-MS for detection and interpretation of compounds present in N. sativa oil samples. Fixed oil was obtained through column chromatography of ethyl acetate fraction. The oil samples were subjected to GC-MS analysis which showed 5, 18, 12 and 20 compounds in four fixed oil samples respectively. The major components were linoleic acid, methyl ester (35.5%), oleic acid, methyl ester (15.007%), palmitic acid, methyl ester (8.208%).

Keywords: column chromatography, fixed oil, GC-MS, Nigella sativa

Author Biography

Sania Feroz, Institute of Chemical Sciences, University of Peshawar, K.P.K Peshawar-25120, Pakistan

 

                          

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How to Cite
Feroz, S., and G. Uddin. “GC-MS ANALYSIS OF FIXED OILS OF NIGELLA SATIVA SEEDS”. Universal Journal of Pharmaceutical Research, Vol. 3, no. 2, May 2018, pp. 38-42, doi:https://doi.org/10.22270/ujpr.v3i2.139.
Section
Research Articles