RIBAVIRIN LOADED ERYTHROCYTES BY ENDOCYTOSIS AS TARGETED DRUG CARRIER SYSTEM

  • Walid Anwar Abdul-Raheem Pharmaceutics Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.
  • Ayman Salama Pharmaceutics Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.
  • Mohsen I Afouna Pharmaceutics Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.
  • Ahmed M Samy Pharmaceutics Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.

Abstract

Objectives: Loaded erythrocytes, as drug carrier system, have tremendous potential to carry outside specificity and sustained release of drug. Thereby, enhancing therapeutic index and minimize the dose and adverse effects as well as improvement patient compliance. In the present paper, erythrocytes loaded Ribavirin with the aim to benefit the reticuloendothelial system targeting potential of the carrier cells.


Methods: Endocytosis technique was used for Ribavirin loading in erythrocytes and the entire loading procedure was evaluated and validated. The in-vitro release of carrier erythrocytes was characterized, as well as the hematological indices, osmotic fragility and SEM analysis.


Results: The maximum loaded amount and entrapment efficiency were found to be 9.58±0.045 mg and 38.3% at 25mg/ml of Ribavirin concentration after 60 minutes incubation time at 37oC with 88.42% cell recovery. The in-vitro Ribavirin release was found to obey Higuchi diffusion kinetics. Hematological parameters of Ribavirin loaded erythrocytes were significantly differ from native erythrocytes at (p≤ 0.01).


Conclusion: The highly changed erythrocyte shape and morphology being one of the main determinants in erythrocytes disappearance kinetics in circulation, can be potentially beneficial in terms of successful cell targeting to the reticulo endothelial system which in turn leads to the improved Ribavirin effects on RES-mediated immune responses.


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Peer Review History:


Article received on- 3 October; Revised on- 22 October; Accepted on- 5 November, Available online 15 November 2016


Academic Editor: Dr. Asia Selman Abdullahorcid22.jpg, Al-Razi university, Department of Pharmacy, Yemen, asia_abdullah65@yahoo.com


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Received file:blue_23983.gif        Reviewer's Comments:download_logo_r_29189.gif


Average Peer review marks at initial stage: 6.5/10


Average Peer review marks at publication stage: 8.0/10


Reviewer(s) detail:


Dr. A.K. Raiorcid22.jpg, PSIT, Kanpur, U.P, India, drawanikrai@gmail.com


Dr. Sally A. El-Zahabyorcid22.jpg, Pharos University in Alexandria, Egypt, sally.elzahaby@yahoo.com

Keywords: Endocytosis, erythrocytes, osmotic fragility and scanning electron microscope

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How to Cite
Abdul-Raheem, W., A. Salama, M. Afouna, and A. Samy. “RIBAVIRIN LOADED ERYTHROCYTES BY ENDOCYTOSIS AS TARGETED DRUG CARRIER SYSTEM”. Universal Journal of Pharmaceutical Research, Vol. 1, no. 1, Sept. 2016, doi:https://doi.org/10.22270/ujpr.v1i1.R6.
Section
Research Articles