ESTIMATION OF ANTI-INFLAMMATORY ACTIVITY AS WELL AS APOPTOTIC ACTIVITY OF ETHANOLIC EXTRACTS OF CROCUS SATIVUS

  • B. Arirudran PG Department of Biochemistry, SRM Arts and Science College, Kanchipuram district, Tamilnadu, India. https://orcid.org/0000-0002-1663-0635
  • P. Priyadharshini PG Department of Biochemistry, SRM Arts and Science College, Kanchipuram district, Tamilnadu, India.
  • US Mahadeva Rao School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin, Kuala Terengganu, 20400 Malaysia. https://orcid.org/0000-0003-4753-0423

Abstract

Inflammation is a body reaction which embroils cellular and biochemical responses, which is not only symptom for shared diseases but also known to be an initial phase for certain serious Alzheimer’s, cancer, heart vascular diseases. In order to overcome these drawbacks, there is an urgent need for nutraceuticals with excellent anti-inflammatory response with minimum side effects. Aim: An attempt has been made to evaluate the anti-inflammatory activity along with gene expression analysis on ethanolic extracts of Crocus sativus (CSEE). Dried stigmas of C. sativus were analyzed for anti-inflammatory activity by macrophage scavenging assay. In this study, the phagocytic activity of the extract was tested on oxidative burst reduction of macrophages. RT-PCR was performed to analyze the anti-apoptotic gene expression during cell death, as a result of the compound treatment on cancer cells. The CSEE unveiled high phagocytic activity on the oxidative burst reduction, presenting intracellular killing and the enhancement of lysosomal enzyme activity, showing the active degranulation of macrophages. These findings suggest that C. sativus possessed excellent anti-inflammatory as well as apoptotic activities. Hence it was proposed that C. sativus could be exploited against oxidative stress, anti-inflammatory, cancer and ageing therapy to justify their use in traditional medicine as a nutraceutical.


Peer Review History:


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Received file


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Average Peer review marks at initial stage: 5.5/10


Average Peer review marks at publication stage: 8.0/10


Reviewer(s) detail:


Name: Dr. Nada Farrag  


Affiliation: Misr International University, Egypt


E-mail: Nada_Hazem87@hotmail.com


 


Name: Noha El Baghdady 


Affiliation: MTI University, Cairo, Egypt


E-mail: nohasalah21@yahoo.com


Comments of reviewer(s):


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Specific Comments:


Overall, this manuscript is good and very interesting to be discussed about nanovesicle including characterization, formulation, and stability test.

Keywords: Anti-inflammatory, crocus sativus, macrophage, nutraceutical, oxidative stress
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How to Cite
Arirudran, B., P. Priyadharshini, and U. M. Rao. “ESTIMATION OF ANTI-INFLAMMATORY ACTIVITY AS WELL AS APOPTOTIC ACTIVITY OF ETHANOLIC EXTRACTS OF CROCUS SATIVUS”. Universal Journal of Pharmaceutical Research, Vol. 3, no. 6, Jan. 2019, doi:https://doi.org/10.22270/ujpr.v3i6.217.
Section
Research Articles