CYTOTOXIC EFFECT AND PHYTOCHEMICAL STUDY OF PETROLEUM ETHER EXTRACT OF TILIA CORDATA MILL
Objective: The aim of this research was to investigate the chemical composition of petroleum ether extract of Tilia cordata aerial parts as well as to evaluate its cytotoxic activity.
Methods: Gas chromatography and gas chromatography–mass spectrometry (GC-MS) were used to analyze the unsaponifiable matter and fatty acid methyl esters. Moreover, the cytotoxicity was examined against human hepatoma HepG2 cell line and breast adenocarcinoma MCF cell line.
Results: The result showed that thirteen compounds were identified in the fatty acid methyl esters fraction representing 93.71% of the total identified peak area. The major compounds were Octadecanoic acid methyl ester (36.26%) and eicosanoic acid methyl ester (29.42%), whereas nineteen compounds in the unsaponifiable fraction were identified representing 90.56 % of the total beak area. The major compounds were 1-Nonene (30.44%), 1-Hexadecene (24.83%) and phytol (10.40%). Moreover, petroleum ether extract showed a potent cytotoxic effect against human hepatoma HepG2 cell line and a moderate cytotoxic effect on breast adenocarcinoma MCF7 human tumor cell line.
Conclusion: So the current research aims to be the first step toward the use of petroleum ether extract of Tilia cordata aerial parts as a potent cytotoxic drug.
Peer Review History:
Received 4 August 2019; Revised 22 August; Accepted 8 September, Available online 15 September 2019
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Average Peer review marks at initial stage: 8/10
Average Peer review marks at publication stage: 9.5/10
Name: Dr. Mohamed Said Fathy Al-Refaey
Affiliation: University of Sadat City, Menofia, Egypt
Name: Dr. Ali Gamal Ahmed Al-kaf
Affiliation: Sana'a University, Yemen
Name: Prof. Dr. Hüsniye Kayalar
Affiliation: Ege University, Turkey
Comments of reviewer(s):
PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF COLOCASIA ESCULENTA (TARO) MEDICINAL PLANT LEAVES USED IN FOLK MEDICINE FOR TREATMENT OF WOUNDS AND BURNS IN HUFASH DISTRICT AL MAHWEET GOVERNORATE–YEMEN