EFFECTS OF RAW AND COOKED AQUEOUS AND METHANOL EXTRACTS OF PHASEOLUS VULGARIS (KIDNEY BEANS) ON RENAL FUNCTION IN ALBINO WISTAR RATS

  • Idoko A Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria. https://orcid.org/0000-0001-7969-1489
  • Philip OC Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria.
  • Nwali ON Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria.
  • Ugwudike PO Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria.
  • Blessing NO Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria.
  • Onyinye AN Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria.
  • Ani PN Department of Microbiology, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria.
  • Ayomide TA Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria. https://orcid.org/0000-0002-2187-9849

Abstract

Objective: Phaseolus vulgaris, like other beans, is endowed with rich nutritional contents. This study evaluated the effects of raw and cooked aqueous and methanol extracts of P. vulgaris on renal function in albino Wistar rats.


Methods: Oral acute toxicity (LD50) study of both extracts was conducted in two phases. In the main design, a total 36 Wistar albino rats were used and divided into nine groups of four rats and oral administration lasted for 7 days. Group 1 served as control and 2 – 9 treated groups. Groups 2 and 3; 4 and 5 were administered aqueous extracts while groups 6 and 7; 8 and 9 were administered methanol extracts of 350mg/kg and 550mg/kg body weight raw and cooked P. vulgaris respectively.


Results: Results of LD50 of all extracts were greater than 5000mg/kg. Results showed a significant (P<0.05) increase in concentrations of urea and chloride across test groups administered aqueous extracts, than methanol extracts; a significant (P<0.05) increase in serum creatinine in test groups administered methanol extracts; a significant (P<0.05) increase of serum total protein of test groups compared to control; no significant (P<0.05) difference in the concentration of potassium in test groups administered compared to control group.


Conclusion: It may be concluded that P. vulgaris portrays potentials capable of improving renal function and its consumption may contribute to the wellness of a person due to its rich nutrients, and based on the duration of this work and standard scale of toxicity; the extracts are practically non- toxic since the LD50 was greater than 5000mg/kg.  


Peer Review History:


Received 7 June 2020; Revised 25 June; Accepted 3 July, Available online 15 July 2020  


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Received file


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Average Peer review marks at initial stage: 6.0/10


Average Peer review marks at publication stage: 8.0/10


Reviewer(s) detail:


Name: Dr. Ali Gamal Ahmed Al-kaf


Affiliation: Sana'a university, Yemen


E-mail: alialkaf21@gmail.com


 


Name: Ahmad Najib


Affiliation: Universitas Muslim Indonesia, Makassar, Indonesia


E-mail: ahmad.najib@umi.ac.id


 


Comments of reviewer(s):


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Keywords: Creatinine, kidney function, Phaseolus vulgaris, potassium chloride, urea
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How to Cite
A, I., P. OC, N. ON, U. PO, B. NO, O. AN, A. PN, and A. TA. “EFFECTS OF RAW AND COOKED AQUEOUS AND METHANOL EXTRACTS OF PHASEOLUS VULGARIS (KIDNEY BEANS) ON RENAL FUNCTION IN ALBINO WISTAR RATS”. Universal Journal of Pharmaceutical Research, Vol. 5, no. 3, July 2020, doi:https://doi.org/10.22270/ujpr.v5i3.408.
Section
Research Articles