CLINICAL APPLICATION OF PLASMA PROTEIN C DETERMINATION

  • George Zhu The Institute of oncology, Tehran University of Medical sciences, Tehran, Iran.
  • AW Broekmans University Hospital Leiden, The Netherlands.
  • RM Bertina University Hospital Leiden, The Netherlands.

Abstract

 Objective: Protein C, a vitamin K-dependent coagulation factor, is involved in blood coagulation. Activated protein C inactivates Va and VIIIa and stimulates fibrinolysis. In this process, protein S serve as an important factor for activated protein C. Furthermore, excess protein S drives cancer cell proliferation and cell survival through oncogenic receptor Axl(Anexelekto). We determined ranges of protein C both in healthy individuals and distinct hospitalized patients.


Methods: A total of 100 patients with different diagnostic diseases and 50 healthy individuals were included in their plasma protein C determination. A rabbit antibody against human protein C was used for the quantitative estimation of plasma protein C antigen by using rocket immunoassay.


Results: In healthy individuals protein C antigen (PC:Ag) ranges o.6439- 1.4752 µ/ml. The mean coefficient of variation (CV) of length of rocket was calculated to be 12.45%. PC:Ag within laboratory variation was 11.47%. Plasma protein C antigen was destroyed at 56℃ for 30 minutes, whereas no significant decrease of protein C was found at 4℃ refrigerator for one week.


Conclusion:  The results showed that plasma protein C antigen was considerably high in 22 diabetes mellitus. On the other hand, the PC:Ag was significantly decreased in 19 liver cirrhosis(p< 0.001) and was positively correlation with serum albumin levels(p< 0.05). In 20 acute leukemias, on the average,there was slightly lower values in PC:Ag, and accompanied with significant decrease of PC:Ag in 5 M5 subtype and in 9 hyper- leukocytes acute leukemias. However, the 3 acute promyelocytic leukemia (APL) with overt laboratory picture of DIC(disseminated intravascular coagulation) had protein C concentration no lower than the remaining 2 patients with infectious DIC, which suggested the coagulopathy in APL might be due to mechanisms different from other forms of DIC.


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Peer Review History:


Received 17 November  2020; Revised 12 Decembe; Accepted 1 January, Available online 15 January 2021


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Received file:blue_23983.gif                Reviewer's Comments:download_logo_r_29189.gif


Average Peer review marks at initial stage: 5.5/10


Average Peer review marks at publication stage: 7.5/10


Reviewer(s) detail:


Dr. Idoko Alexanderorcid22.jpg,


Caritas University, Enugu, Nigeriaidokoalexander1@gmail.com


Dr. Rawaa Souhil Al-Kayaliorcid22.jpg,


Aleppo University, Syriarawah67@hotmail.com


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Keywords: Antigen, clinical determination, plasma protein C, rocket immunoassay

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How to Cite
Zhu, G., A. Broekmans, and R. Bertina. “CLINICAL APPLICATION OF PLASMA PROTEIN C DETERMINATION”. Universal Journal of Pharmaceutical Research, Vol. 5, no. 6, Jan. 2021, doi:https://doi.org/10.22270/ujpr.v5i6.509.
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Research Articles