ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF OMEPRAZOLE AND ASPIRIN USING REVERSE PHASE HPLC METHOD IN BULK AND DOSAGE FORM
A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Aspirin and Omeprazole in bulk and pharmaceutical dosage form using C18 column (Agilent, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of Methanol and 0.1 M Dipotassium Phosphate buffer (pH 3) in the ratio of 60:40 v/v. The detection was carried out at 256 nm. The method was linear over the concentration range for Omeprazole 50-250 μg/ml and for Aspirin 10-50 μg/ml. The recoveries of Omeprazole and Aspirin were found to be 100.07 and 100.06% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.
Peer Review History:
Received 22 August; Revised 28 August; Accepted 5 September, Available online 15 September 2017
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Average Peer review marks at initial stage: 5.0/10
Average Peer review marks at publication stage: 7.5/10
Name: Dr. Showkat Ahmad Bhawani
Affiliation: UNIMAS Sarawak, Malaysia
Name: Dr. Maya Shaaban Eissa Hussein
Affiliation: Egyptian Russian University, Egypt
Comments of reviewer(s):