EVALUATION OF ANTIBACTERIAL RESISTANCE OF BIOFILM FORMS OF AVIAN SALMONELLA GALLINARUM TO FLUOROQUINOLONES

Kumar Kamashi; Mr. Honnegowda; Mayanna Asha; Chandrakala Ms.

doi:10.22270/ujpr.v3i4.175

Kumar Kamashi Anatomy, Physiology and Pharmacology Academic Program, School of Veterinary Medicine, St. George’s University, Grenada, West Indies.
Mr. Honnegowda Department of Pharmacology and Toxicology, Veterinary College, University of Agricultural Sciences, Hebbal, Bangalore, India
Mayanna Asha Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore, India.
Chandrakala Ms. Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore, India.

10.22270/ujpr.v3i4.175

Keywords:

Antibiotic resistance, biofilm, biofilm elimination concentration (BEC), fluoroquinolones, minimum bactericidal concentration (MBC), minimum inhibitory concentration (MIC), S. gallinarum

Abstract

Objective: Antimicrobial resistance is a growing concern worldwide. The indiscriminate use of antibiotics for a period of time has led to the emergence of antibiotic resistance in pathogenic bacteria. The present study was designed to evaluate the antibacterial efficacy of fluoroquinolone drugs, ciprofloxacin, enrofloxacin, moxifloxacin, sparfloxacin, norfloxacin, pefloxacin and ofloxacin against avian Salmonella gallinarum bacterial biofilms.

Methods: The study parameters, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and biofilm elimination concentration (BEC) were determined on days 1, 3, 7, 10, 14 and 20 post inoculation for the planktonic (free) and biofilm cells of S. gallinarum by macro broth dilution method. The MIC and MBC values determined on days 1, 3, 7, 10, 14 and 20 for each of the fluoroquinolone drugs against the planktonic and biofilm forms of avian S. gallinarum were found to be non-significant.

Results: BEC values determined against the biofilm forms of S. gallinarum during the study period were found to be non-significant among the tested fluoroquinolones.

Conclusion: The results of the present study demonstrated that fluoroquinolone drugs were effective in vitro against both the planktonic and biofilm forms of avian S. gallinarum.