ANTIMICROBIAL ACTIVITY OF SODIUM HYPOCHLORITE, NANO SILVER AND CHLORHEXIDINE AGAINST MONO-SPECIES BIOFILMS OF SELECTED MICROORGANISMS OF ORAL SOURCES

  • Mohammed Mohammed Ali Alsamhari Department of conservative dentistry, Faculty of Dentistry, Genius University for Sciences & Technology, Dhamar city, Republic of Yemen.
  • Mohammed Mohammed Lutf AlKhawlani Department of conservative dentistry, Faculty of Dentistry, Genius University for Sciences & Technology, Dhamar city, Republic of Yemen.
  • Abdulwahab Ismail Mohamed Al-Kholani Department of conservative dentistry, Faculty of Dentistry, Sana’a University, Republic of Yemen.
  • Mohammed Mohammed Ali Al-Najhi Department of conservative dentistry, Faculty of Dentistry, Sana’a University, Republic of Yemen.
  • Hassan Abdulwahab Al-Shamahy Departement of Basic Sciences, Faculty of Dentistry, Sana’a University, Republic of Yemen. Medical Microbiology department, Faculty of Medicine, Genius University for Sciences & Technology, Dhamar city, Republic of Yemen.
  • Amani Abdulhakeem Al-Sharani Oral Medicine and Periodontology Department, College of Dentistry, University of Sciences and Technology, Yemen.
  • Omar Ahmed Ismael Al-dossary Departement of Basic Sciences, Faculty of Dentistry, Sana’a University, Republic of Yemen.
  • Khaled A AL-Haddad Medical Microbiology department, Faculty of Medicine, Genius University for Sciences & Technology, Dhamar city, Republic of Yemen.
  • Mohammed A Al-labani Medical Microbiology department, Faculty of Medicine, Genius University for Sciences & Technology, Dhamar city, Republic of Yemen.
10.22270/ujpr.v7i1.716

Keywords:

Candida albicans, Chlorhexidine, Endodontic-irrigating, Enterococcus faecalis, Nanosilver, Pseudomonas aeruginosa, Sodium hypochlorite, S. aureus

Abstract

Background: Among the measures involved in the control of endodontic infection, irrigation is an important factor in the elimination of microorganisms present in the open root canal. It is known that mechanical debridement alone does not result in a complete or permanent reduction of bacteria, therefore antimicrobial agents are used as an aid to mechanical cleaning to completely kill or reduce the number of microorganisms.

Aim:  To investigate the antimicrobial activity of 2.5%, 5.25% sodium hypochlorite and 2.0% chlorhexidine liquid and as the first new study of 60 mg/L silver nanoparticles as an endodontic-irrigating substances against single-species biofilms.

Methods: Biofilms of Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans oral sources were produced on a cellulose nitrate membrane placed on agar medium. Membrane filters were transferred to tubes containing 2 mL of fresh broth medium plus neutralizers. Microorganisms were suspended using a vortex, and the inoculum was serially diluted 10-fold. Aliquots of the dilutions were placed on 5% blood agar medium and incubated under appropriate gaseous conditions. Colony forming units were calculated.

Results:  The antimicrobial agents in liquid presentation, especially 5.25% NaOCl, 2% chlorhexidine, and 2% nano-silver liquid killed the tested microorganisms more rapidly with mean rank equal to 1.4, 1.9, and 1.6, respectively. Saline did not inhibit the growth of any of the tested microorganisms, as it was statistically (p < 0.05) different for NaOCl, Nano-silver and chlorhexidine.

Conclusions:  Preferring agents in liquid supply to get rid of biofilm microorganisms, especially 5.25% NaOCl and 60mg/L nanosilver liquid.

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Peer Review History:

Received: 24 December 2021; Revised: 19 January 2022; Accepted: 27 February, Available online: 15 March 2022

Academic Editor: Dr. Nuray Arıorcid22.jpg, Ankara University, Turkiye, ari@ankara.edu.tr

Received file:blue_23983.gif                Reviewer's Comments:download_logo_r_29189.gif

Average Peer review marks at initial stage: 6.5/10

Average Peer review marks at publication stage: 7.5/10

Reviewers:

orcid22.jpgProf. Dr. Gorkem Dulger, Duzce University, Turkey, gorkemdulger@yandex.com

orcid22.jpgDr. Rawaa Souhil Al-Kayali, Aleppo University, Syriarawah67@hotmail.com

orcid22.jpgDr. Wadhah Hassan Ali Edrees, Hajja University, Yemen, edress2020@gmail.com

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Published

2022-03-15

How to Cite

Alsamhari, M. M. A., M. M. L. AlKhawlani, A. I. M. Al-Kholani, M. M. A. Al-Najhi, H. A. Al-Shamahy, A. A. Al-Sharani, O. A. I. Al-dossary, K. A. AL-Haddad, and M. A. Al-labani. “ANTIMICROBIAL ACTIVITY OF SODIUM HYPOCHLORITE, NANO SILVER AND CHLORHEXIDINE AGAINST MONO-SPECIES BIOFILMS OF SELECTED MICROORGANISMS OF ORAL SOURCES”. Universal Journal of Pharmaceutical Research, vol. 7, no. 1, Mar. 2022, doi:10.22270/ujpr.v7i1.716.

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